Video Tutorials

Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. This course covers: -Overview of a basic flow cytometry staining protocol for cell surface markers -Fc block -Five factors that affect antibody staining -Viability dyes -Fixation Download the slides: https://voices.uchicago.edu/ucflow/project/flow-cytometry-basic-training-course-2/

Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. This course covers: -Optimizing tissue digestion -Other blocking options -Determining the number of cells to stain -Antibody titration Download the slides: https://voices.uchicago.edu/ucflow/project/flow-cytometry-basic-training-course-2/

Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. This course covers: -Information to gather beforehand -Tools for panel design -How to assign markers to fluorophores Download the slides: https://voices.uchicago.edu/ucflow/project/flow-cytometry-basic-training-course-2/

Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. This course covers: Controls (Single Stain, FMO, Isotype, Isoclonic) How to divide cells between controls Download the slides: https://voices.uchicago.edu/ucflow/project/flow-cytometry-basic-training-course-2/ How to pipette FMOs: https://youtu.be/9HXIRZf6rzw

Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. This course covers: -Organizing the experimental layout (1:37) -Setting Voltages (13:05) -Automated Compensation (29:34) -How to identify compensation errors Download the slides: https://voices.uchicago.edu/ucflow/project/flow-cytometry-basic-training-course-2/